Correlation Between Pancreatic Islet Uncoupling Protein-2 (UCP2) mRNA Concentration And Insulin Status in Rats | Zendy

Nadim Kassis | Zendy; Catherine Bernard | Zendy; A. Pusterla | Zendy; Louis Casteilla | Zendy; Luc Pétnicaud | Zendy; Denis Richard | Zendy; Daniel Ricquier | Zendy; Alain Ktorza | Zendy

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Correlation Between Pancreatic Islet Uncoupling Protein-2 (UCP2) mRNA Concentration And Insulin Status in Rats

Author(s) -

Nadim Kassis,

Catherine Bernard,

A. Pusterla,

Louis Casteilla,

Luc Pétnicaud,

Denis Richard,

Daniel Ricquier,

Alain Ktorza

Publication year - 2000

Publication title -

journal of diabetes research

Language(s) - English

Resource type - Journals

eISSN - 2314-6753

pISSN - 2314-6745

DOI - 10.1155/edr.2000.185

Subject(s) - endocrinology , medicine , hyperinsulinemia , insulin , islet , streptozotocin , uncoupling protein , pancreatic hormone , diabetes mellitus , insulin oscillation , gene expression , pancreatic islets , messenger rna , biology , chemistry , insulin resistance , adipose tissue , gene , brown adipose tissue , biochemistry

Hypothesizing that UCP2 may influence insulin secretion by modifying the ATP/ADP ratio within pancreatic islets, we have investigated the expression of intraislet UCP2 gene in rats showing insulin oversecretion (non-diabetic Zucker fa/fa obese rats, glucose-infused Wistar rats) or insulin undersecretion (fasting and mildly diabetic rats). We found that in Zucker fa/fa obese rats, hyperinsulinemia (1222+/-98 pmol/l vs. 128+/-22 pmol/l in lean Zucker rats) was accompanied by a significant increase in UCP2 mRNA levels. In rat submitted to a 5 day infusion with glucose, hyperinsulinemia (1126+/-101 pmol/l vs. 215+/-25 pmol/l in Wistar control rats), coincided with an enhanced intraislet UCP2 gene expression, whereas a 8h or a 2 day-infusion did not induce significant changes in UCP2 mRNA expression. In rats made hypoinsulinemic and mildly diabetic by the injection of a low dose of streptozotocin, and in 4-day-fasting rats (plasma insulin 28+/-5 pmol/l) UCP2 gene expression was sharply decreased. A 3-day-fast was ineffective. The data show the existence of a time-dependent correlation between islet mRNA UCP2 and insulin that may be interpreted as an adaptative response to prolonged insulin excess.

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