Inhibitory Effect of Diabetes on Proliferation of Vascular Smooth Muscle After Balloon Injury in Rat Aorta | Zendy

Gunilla Dahlfors | Zendy; Yun Chen | Zendy; Bertil Gustafsson | Zendy; Hans J. Arnqvist | Zendy

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Inhibitory Effect of Diabetes on Proliferation of Vascular Smooth Muscle After Balloon Injury in Rat Aorta

Author(s) -

Gunilla Dahlfors,

Yun Chen,

Bertil Gustafsson,

Hans J. Arnqvist

Publication year - 2000

Publication title -

journal of diabetes research

Language(s) - English

Resource type - Journals

eISSN - 2314-6753

pISSN - 2314-6745

DOI - 10.1155/edr.2000.101

Subject(s) - vascular smooth muscle , bromodeoxyuridine , endocrinology , diabetes mellitus , medicine , transforming growth factor , streptozotocin , aorta , messenger rna , cell growth , in situ hybridization , in vivo , gene expression , biology , smooth muscle , gene , immunohistochemistry , biochemistry , microbiology and biotechnology

The effect of streptozotocin-induced diabetes on cell proliferation in rat aortic intima-media, as well as on local gene expression of transforming growth factor-beta1 (TGF-beta1) was studied. TGF-beta1 mRNA was measured by solution hybridization and TGF-beta1 protein by ELISA. Proliferation was measured by bromodeoxyuridine incorporation into DNA two days after balloon injury. All BrdU-labelled cells observed were smooth muscle cells. After a diabetes duration of 2 and 4 weeks, labelled cells were significantly fewer compared with controls. Circulating levels of total TGF-beta1 were lowered in rats with 2 weeks diabetes. Although the balloon injury procedure by itself stimulated the gene expression of TGF-beta1, no significant difference in TGF-beta1 mRNA content between diabetic and control rats after injury was found.

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