Open AccessInterleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells
Author(s) -
Bruna Cristina Falavinha,
María Julia Barisón,
Cármen Lúcia Kuniyoshi Rebelatto,
Bruna Hilzendeger Marcon,
Alessandra Melo de Aguiar,
Evelin Brandão da Silva,
Marco Augusto Stimamiglio,
Patrícia Shigunov
Publication year - 2022
Publication title -
stem cells international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.205
H-Index - 64
eISSN - 1687-9678
pISSN - 1687-966X
DOI - 10.1155/2022/4930932
Subject(s) - adipogenesis , adipose tissue , stromal cell , stem cell , adipocyte , microbiology and biotechnology , gene silencing , mesenchymal stem cell , biology , stromal vascular fraction , receptor , cancer research , endocrinology , biochemistry , gene
Dysfunctions in adipose tissue cells are responsible for several obesity-related metabolic diseases. Understanding the process of adipocyte formation is thus fundamental for understanding these diseases. The adipocyte differentiation of adipose-derived stem/stromal cells (ADSCs) showed a reduction in the mRNA level of the interleukin 21 receptor (IL21R) during this process. Although the receptor has been associated with metabolic diseases, few studies have examined its function in stem cells. In this study, we used confocal immunofluorescence assays to determine that IL21R colocalizes with mitochondrial protein ATP5B, ALDH4A1, and the nucleus of human ADSCs. We demonstrated that silencing and overexpression of IL21R did not affect the cell proliferation and mitochondrial activity of ADSCs. However, IL21R silencing did reduce ADSC adipogenic capacity. Further studies are needed to understand the mechanism involved between IL21R and the adipogenic differentiation process.
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