SNHG1/miR-145-5p/KLF5 Axis Participates in Regulating the Proliferation and Migration of Oral Squamous Cell Cancer | Zendy

Haiyan Xin | Zendy; Ziyan Feng | Zendy; Chao Yao | Zendy

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SNHG1/miR-145-5p/KLF5 Axis Participates in Regulating the Proliferation and Migration of Oral Squamous Cell Cancer

Author(s) -

Haiyan Xin,

Ziyan Feng,

Chao Yao

Publication year - 2022

Publication title -

journal of healthcare engineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 29

eISSN - 2040-2309

pISSN - 2040-2295

DOI - 10.1155/2022/2053271

Subject(s) - cancer research , downregulation and upregulation , oncogene , cell , cancer , biology , microrna , cell growth , gene , reporter gene , microbiology and biotechnology , mutant , squamous cell cancer , gene expression , genetics , cell cycle

We aimed to clarify the molecular mechanism of lncRNA SNHG1 in regulating the OSCC process. Clinical samples of OSCC were collected for detecting the differential level of SNHG1 by qRT-PCR. Pathological indexes of OSCC patients were analyzed for uncovering the prognostic value of SNHG1. The interaction between SNHG1 and miR-145-5p was assessed through the bioinformatics method and dual-luciferase reporter assay. Their coregulation on proliferative and migratory functions of Tca8113 and CAL-27 cells was explored by the CCK-8, EdU, and Transwell assay. Finally, the regulatory effect of miR-145-5p on its downstream gene KLF5 was evaluated. SNHG1 was abnormally upregulated in OSCC samples and linked to a poor prognosis of OSCC patients. Serving as an oncogene, SNHG1 strengthened proliferative and migratory functions of Tca8113 and CAL-27 cells. miR-145-5p was a key downstream target inducing the oncogenic role of SNHG1 in the OSCC process with KLF5 as its downstream gene. SNHG1/miR-145-5p/KLF1 axis is responsible for driving the malignant process of OSCC.

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