Transcriptome Characterization and Identification of Molecular Markers (SNP, SSR, and Indels) in the Medicinal Plant Sarcandra glabra spp. | Zendy

Yanqin Xu | Zendy; Shuyun Tian | Zendy; Renqing Li | Zendy; Xiaofang Huang | Zendy; Fengqin Li | Zendy; Fei Ge | Zendy; Wenzhen Huang | Zendy; Yin Zhou | Zendy

Open Access

Transcriptome Characterization and Identification of Molecular Markers (SNP, SSR, and Indels) in the Medicinal Plant Sarcandra glabra spp.

Author(s) -

Yanqin Xu,

Shuyun Tian,

Renqing Li,

Xiaofang Huang,

Fengqin Li,

Fei Ge,

Wenzhen Huang,

Yin Zhou

Publication year - 2021

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2021/9990910

Subject(s) - indel , biology , subspecies , transcriptome , genetics , molecular marker , gene , molecular breeding , single nucleotide polymorphism , snp , microsatellite , identification (biology) , botany , gene expression , allele , genotype , paleontology

Sarcandra glabra has significant metabolically active bioingredients of pharmaceutical importance. The deficiency of molecular markers for S. glabra is a hindrance in molecular breeding for genetic improvement. In this study, 57.756 million pair-end reads were generated by transcriptome sequencing in S. glabra (Thunb.) Nakai and its subspecies S. glabra ssp. brachystachys . A total of 141,954 unigenes with 646.63 bp average length were assembled. A total of 25,620 simple sequence repeats, 726,476 single nucleotide polymorphisms, and 42,939 insertions and deletions were identified, and the associated unigenes and differentially expressed genes were characterized. This work enhanced the molecular marker resources and will facilitate molecular breeding and gene mining in S. glabra spp.

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