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Probiotics are widely used for protection against stress-induced intestinal dysfunction. Oxidative stress plays a critical role in gastrointestinal disorders. It is established that probiotics alleviate oxidative stress; however, the mechanism of action has not been elucidated. We developed an  in vitro  intestinal porcine epithelial cells (IPEC-J2) model of oxidative stress to explore the antioxidant effect and potential mode of action of  Lactobacillus plantarum  ZLP001. The IPEC-J2 cells were preincubated with and without  L. plantarum  ZLP001 for 3 h and then exposed to hydrogen peroxide (H 2 O 2 ) for 4 h. Pretreatment with  L. plantarum  ZLP001 protected IPEC-J2 cells against H 2 O 2 -induced oxidative damage as indicated by cell viability assays and significantly alleviated apoptosis elicited by H 2 O 2 .  L. plantarum  ZLP001 pretreatment decreased reactive oxygen species production and the cellular malondialdehyde concentration and increased the mitochondrial membrane potential compared with H 2 O 2  treatment alone, suggesting that  L. plantarum  ZLP001 promotes the maintenance of redox homeostasis in the cells. Furthermore,  L. plantarum  ZLP001 regulated the expression and generation of some antioxidant enzymes, thereby activating the antioxidant defense system. Treatment with  L. plantarum  ZLP001 led to nuclear erythroid 2-related factor 2 (Nrf2) enrichment in the nucleus compared with H 2 O 2  treatment alone. Knockdown of Nrf2 significantly weakened the alleviating effect of  L. plantarum  ZLP001 on antioxidant stress in IPEC-J2 cells, suggesting that Nrf2 is involved in the antioxidative effect of  L. plantarum  ZLP001. Collectively, these results indicate that  L. plantarum  ZLP001 is a promising probiotic bacterium that can potentially alleviate oxidative stress.

Lactobacillus plantarum Exhibits Antioxidant and Cytoprotective Activities in Porcine Intestinal Epithelial Cells Exposed to Hydrogen Peroxide