English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Candida albicans  and  Candida tropicalis  are the leading causes of human fungal infections worldwide. There is an increase in resistance of  Candida  pathogens to existing antifungal drugs leading to a need to find new sources of antifungal agents. Tormentic acid has been isolated from different plants including  Callistemon citrinus  and has been found to possess antimicrobial properties, including antifungal activity. The study aimed to determine the effects of tormentic and extracts from  C. citrinus  on  C. albicans  and  C .  tropicalis  and a possible mode of action. The extracts and tormentic acid were screened for antifungal activity using the broth microdilution method. The growth of both species was inhibited by the extracts, and  C. albicans  was more susceptible to the extract compared to  C .  tropicalis . The growth of  C .  albicans  was inhibited by 80% at 100  μ g/ml of both the DCM: methanol extract and the ethanol: water extract. Tormentic acid reduced the growth of  C. albicans  by 72% at 100  μ g/ml. The effects of the extracts and tormentic acid on ergosterol content in  C. albicans  were determined using a UV/Vis scanning spectrophotometer. At concentrations of tormentic acid of 25  μ g/ml, 50  μ g/ml, 100  μ g/ml, and 200  μ g/ml, the content of ergosterol was decreased by 22%, 36%, 48%, and 78%, respectively. Similarly, the DCM: methanol extract at 100  μ g/ml and 200  μ g/ml decreased the content by 78% and 88%, respectively. A dose-dependent decrease in ergosterol content was observed in cells exposed to miconazole with a 25  μ g/ml concentration causing a 100% decrease in ergosterol content. Therefore, tormentic acid inhibits the synthesis of ergosterol in  C. albicans . Modifications of the structure of tormentic acid to increase its antifungal potency may be explored in further studies.

the scientific world journal/thescientificworldjournal

The Effects of Tormentic Acid and Extracts from Callistemon citrinus on Candida albicans and Candida tropicalis Growth and Inhibition of Ergosterol Biosynthesis in Candida albicans