Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells | Zendy

Salam SalloumAsfar | Zendy; Rudolf Engelke | Zendy; Hanaa Mousa | Zendy; Neha Goswami | Zendy; I. Richard Thompson | Zendy; Freshteh Palangi | Zendy; Kamal Kamal | Zendy; Mu. Al-Noubi | Zendy; Frank Schmidt | Zendy; Sara Abdulla | Zendy; Mohamed M. Emara | Zendy

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Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells

Author(s) -

Salam SalloumAsfar,

Rudolf Engelke,

Hanaa Mousa,

Neha Goswami,

I. Richard Thompson,

Freshteh Palangi,

Kamal Kamal,

Mu. Al-Noubi,

Frank Schmidt,

Sara Abdulla,

Mohamed M. Emara

Publication year - 2021

Publication title -

stem cells international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.205

H-Index - 64

eISSN - 1687-9678

pISSN - 1687-966X

DOI - 10.1155/2021/8274936

Subject(s) - stress granule , microbiology and biotechnology , ribonucleoprotein , induced pluripotent stem cell , embryonic stem cell , biology , translation (biology) , osmotic shock , stem cell , rna binding protein , cell culture , translational regulation , osmotic concentration , messenger rna , rna , biochemistry , genetics , gene

Stress granules (SGs) are assemblies of selective messenger RNAs (mRNAs), translation factors, and RNA-binding proteins in small untranslated messenger ribonucleoprotein (mRNP) complexes in the cytoplasm. Evidence indicates that different types of cells have shown different mechanisms to respond to stress and the formation of SGs. In the present work, we investigated how human-induced pluripotent stem cells (hiPSCs/IMR90-1) overcome hyperosmotic stress compared to a cell line that does not harbor pluripotent characteristics (SH-SY5Y cell line). Gradient concentrations of NaCl showed a different pattern of SG formation between hiPSCs/IMR90-1 and the nonpluripotent cell line SH-SY5Y. Other pluripotent stem cell lines (hiPSCs/CRTD5 and hESCs/H9 (human embryonic stem cell line)) as well as nonpluripotent cell lines (BHK-21 and MCF-7) were used to confirm this phenomenon. Moreover, the formation of hyperosmotic SGs in hiPSCs/IMR90-1 was independent of eIF2 α phosphorylation and was associated with low apoptosis levels. In addition, a comprehensive proteomics analysis was performed to identify proteins involved in regulating this specific pattern of hyperosmotic SG formation in hiPSCs/IMR90-1. We found possible implications of microtubule organization on the response to hyperosmotic stress in hiPSCs/IMR90-1. We have also unveiled a reduced expression of tubulin that may protect cells against hyperosmolarity stress while inhibiting SG formation without affecting stem cell self-renewal and pluripotency. Our observations may provide a possible cellular mechanism to better understand SG dynamics in pluripotent stem cells.

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