Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model | Zendy

Eetmad A. Arafat | Zendy; Rehab Elsayed Marzouk | Zendy; Sally Abdallah Mostafa | Zendy; Walaa H. E. Hamed | Zendy

Open Access

Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model

Author(s) -

Eetmad A. Arafat,

Rehab Elsayed Marzouk,

Sally Abdallah Mostafa,

Walaa H. E. Hamed

Publication year - 2021

Publication title -

mediators of inflammation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.37

H-Index - 97

eISSN - 1466-1861

pISSN - 0962-9351

DOI - 10.1155/2021/7534601

Subject(s) - ulcerative colitis , immunohistochemistry , pathology , colitis , pathogenesis , tumor necrosis factor alpha , medicine , vimentin , interleukin , inflammatory bowel disease , gastroenterology , biology , cytokine , disease

Background Telocytes (TCs) are a distinct type of interstitial cells that play a vital role in the pathogenesis of ulcerative colitis and colonic tissue hemostasis. The aim of this study was to examine the effect of nanocurcumin (NC) on the morphometric and immunohistochemical characterization of TCs in the ulcerative colitis (UC) rat model.Methods Forty rats were randomly divided into control, NC, UC, and UC+NC groups. At the end of the experiment, the colon was dissected and prepared for histopathological and immunohistochemical assessment. Tissue homogenates were prepared for real-time PCR assessment of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF- α ), and transforming growth factor-beta (TGF- β ) gene expression. Our results revealed extensive mucosal damage with inflammatory cell infiltration, significant reduction of CD34, and vimentin immunostained TCs in the colon of the UC group with significant elevation of expression of IL-6, TNF- α , and TGF- β . The UC+NC-treated group revealed significant elevation of TC count compared to the UC group besides, a significant reduction of the three gene expression.Conclusion NC successfully targeted the colonic tissue, improved the mucosal lesion, preserve TCs distribution, and count through its anti-inflammatory and fibrinolytic properties.

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