Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy | Zendy

Michaela Turčanová | Zendy; Martin Hrtoň | Zendy; Petr Dvořák | Zendy; Kamil Novák | Zendy; Markéta Hermanová | Zendy; Zdeněk Bednařík | Zendy; Stanislav Polzer | Zendy; Jiří Burša | Zendy

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Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Author(s) -

Michaela Turčanová,

Martin Hrtoň,

Petr Dvořák,

Kamil Novák,

Markéta Hermanová,

Zdeněk Bednařík,

Stanislav Polzer,

Jiří Burša

Publication year - 2021

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2021/6879765

Subject(s) - polarizer , materials science , microscopy , polarized light microscopy , collagen fiber , optics , biomedical engineering , pixel , orientation (vector space) , optical microscope , planar , perpendicular , achilles tendon , fiber , tendon , computer science , scanning electron microscope , anatomy , birefringence , physics , mathematics , medicine , composite material , geometry , computer graphics (images)

A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·10 5 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.

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