Effects of Fluorine on Neutrophil Extracellular Trap Formation through Regulating AMPK/p38 Signaling Pathway | Zendy

Yanyan Song | Zendy; Yue Zhang | Zendy; Peijun Zhang | Zendy; Peng Yu | Zendy; Xinchi Shang | Zendy; Yuting Lu | Zendy; Yuehong Li | Zendy; Hang Gao | Zendy

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Effects of Fluorine on Neutrophil Extracellular Trap Formation through Regulating AMPK/p38 Signaling Pathway

Author(s) -

Yanyan Song,

Yue Zhang,

Peijun Zhang,

Peng Yu,

Xinchi Shang,

Yuting Lu,

Yuehong Li,

Hang Gao

Publication year - 2021

Publication title -

oxidative medicine and cellular longevity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.494

H-Index - 93

eISSN - 1942-0900

pISSN - 1942-0994

DOI - 10.1155/2021/6693921

Subject(s) - neutrophil extracellular traps , microbiology and biotechnology , ampk , extracellular , chemistry , trap (plumbing) , signal transduction , p38 mitogen activated protein kinases , inflammation , mapk/erk pathway , biology , phosphorylation , immunology , protein kinase a , physics , meteorology

Fluorine is an important trace element that is widely dispersed, and studies showed that fluorine could cause severe toxicity to fish. The aim of this study was to investigate the effects of fluorine on neutrophil extracellular trap (NET) formation in common carp and clarify the possible mechanism. The neutrophils were isolated and exposed to 0.25, 0.5, or 1 mM sodium fluoride (NaF). The results showed that NaF could induce the formation of NETs which exhibited a DNA-based network structure modified with histones and myeloperoxidase (MPO). Furthermore, NaF led to the production of reactive oxygen species (ROS) in neutrophils. Western blot results showed that NaF significantly increased the phosphorylation of AMPK and p38. In addition, our results showed that NaF-induced NET formation could be inhibited by an AMPK or p38 inhibitor. In conclusion, our results showed that NaF induced NET formation in neutrophils through regulation of the AMPK/p38 signaling pathway.

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