Genotyping by Sequencing-Based Discovery of SNP Markers and Construction of Linkage Map from F5 Population of Pepper with Contrasting Powdery Mildew Resistance Trait | Zendy

Abinaya Manivannan | Zendy; Sena Choi | Zendy; TaeHwan Jun | Zendy; Eun-Young Yang | Zendy; JinHee Kim | Zendy; Eun-Su Lee | Zendy; HyeEun Lee | Zendy; DoSun Kim | Zendy; YulKyun Ahn | Zendy

Open Access

Genotyping by Sequencing-Based Discovery of SNP Markers and Construction of Linkage Map from F5 Population of Pepper with Contrasting Powdery Mildew Resistance Trait

Author(s) -

Abinaya Manivannan,

Sena Choi,

TaeHwan Jun,

Eun-Young Yang,

JinHee Kim,

Eun-Su Lee,

HyeEun Lee,

DoSun Kim,

YulKyun Ahn

Publication year - 2021

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2021/6673010

Subject(s) - powdery mildew , biology , quantitative trait locus , genetics , pepper , genotyping , single nucleotide polymorphism , genetic linkage , marker assisted selection , population , blumeria graminis , snp , snp genotyping , plant disease resistance , tag snp , genetic marker , gene mapping , molecular breeding , genotype , chromosome , horticulture , gene , demography , sociology

Powdery mildew (PM) is a common fungal disease infecting pepper plants worldwide. Molecular breeding of pepper cultivars with powdery mildew resistance is desirable for the economic improvement of pepper cultivation. In the present study, 188 F 5 population derived from AR1 (PM resistant) and TF68 (PM sensitive) parents were subjected to high-throughput genotyping by sequencing (GBS) for the identification of single nucleotide polymorphism (SNP) markers. Further, the identified SNP markers were utilized for the construction of genetic linkage map and QTL analysis. Overall read mapping percentage of 87.29% was achieved in this study with the total length of mapped region ranging from 2,956,730 to 25,537,525 bp. A total of 41,111 polymorphic SNPs were identified, and a final of 1,841 SNPs were filtered for the construction of a linkage map. A total of 12 linkage groups were constructed corresponding to each chromosome with 1,308 SNP markers with the map length of 2506.8 cM. Further, two QTLs such as Pm-2.1 and Pm-5.1 were identified in chromosomes 2 and 5, respectively, for the PM resistance. Overall, the outcomes of the present endeavor can be utilized for the marker-assisted selection of pepper with powdery mildew-resistant trait.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research