Effect of Stem Cell-Derived Extracellular Vesicles on Damaged Human Corneal Endothelial Cells | Zendy

Raffaele Nuzzi | Zendy; Lola Buono | Zendy; Simona Scalabrin | Zendy; Marco De Iuliis | Zendy; Benedetta Bussolati | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Effect of Stem Cell-Derived Extracellular Vesicles on Damaged Human Corneal Endothelial Cells

Author(s) -

Raffaele Nuzzi,

Lola Buono,

Simona Scalabrin,

Marco De Iuliis,

Benedetta Bussolati

Publication year - 2021

Publication title -

stem cells international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.205

H-Index - 64

eISSN - 1687-9678

pISSN - 1687-966X

DOI - 10.1155/2021/6644463

Subject(s) - extracellular vesicles , microbiology and biotechnology , vesicle , stem cell , extracellular , endothelial stem cell , cell , chemistry , biology , biochemistry , in vitro , membrane

Purpose Human corneal endothelial cells (HCECs) are essential to visual function; however, since they have limited proliferative capacity in vivo , they are prone to corneal endothelial dysfunction. At present, the only treatment is a corneal transplantation from donor cadavers. Also, due to a global shortage of donor corneas, it is important to find alternative strategies. Recent studies highlight that stem cell–derived extracellular vesicles (EVs) play a relevant role in stem cell-induced regeneration by reprogramming injured cells and inducing proregenerative pathways. The aim of this work is to evaluate whether EVs derived from mesenchymal stem cells (MSC-EVs) are able to promote regeneration of damaged HCECs.Methods We isolated HCECs from discarded corneas in patients undergoing corneal transplantation or enucleation ( N = 23 patients). Bone marrow mesenchymal stem cells (MSCs) were obtained from Lonza, cultured, and characterized. MSC-EVs were obtained from supernatants of MSCs. In order to establish a valid in vitro damage model to test the regenerative potential of EVs on HCECs, we evaluated the proliferation rate and the apoptosis after exposing the cells to serum-deprived medium at different concentrations for 24 hours. We then evaluated the HCEC migration through a wound healing assay.Results In the selected serum deprivation damage conditions, the treatment with different doses of MSC-EVs resulted in a significantly higher proliferation rate of HCECs at all the tested concentrations of EVs (5‐20 × 10 3 MSC-EV/cell). MSC-EVs/cell induced a significant decrease in number of total apoptotic cells after 24 hours of serum deprivation. Finally, the wound healing assay showed a significantly faster repair of the wound after HCEC treatment with MSC-EVs.Conclusions Results highlight the already well-known proregenerative potential of MSC-EVs in a totally new biological model, the endothelium of the cornea. MSC-EVs, indeed, induced proliferation and survival of HCECs, promoting the migration of HCECs in vitro .

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research