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Cerium Oxide/Polypyrrole Nanocomposite as the Matrix for Cholesterol Biosensor
Author(s) -
Ta Thi Nhat Anh,
Vu Van Thu,
Hai-Son Dang,
VuongHung Pham,
Phuong Dinh Tam
Publication year - 2021
Publication title -
advances in polymer technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 44
eISSN - 1098-2329
pISSN - 0730-6679
DOI - 10.1155/2021/6627645
Subject(s) - polypyrrole , materials science , cholesterol oxidase , nanocomposite , cerium oxide , cerium , biosensor , electrode , nuclear chemistry , oxide , chemical engineering , nanotechnology , organic chemistry , polymer , chemistry , composite material , polymerization , engineering , metallurgy , enzyme
A nanocomposite comprising cerium oxide nanoparticles (CeO2 NPs) and polypyrrole (PPy) was fabricated onto a microelectrode for cholesterol sensing application. The cholesterol oxidase (ChOx) was immobilized on the CeO2 NPs/PPy/electrode by the physical adsorption route. The structure and morphology of the CeO2 NPs/PPy nanocomposite were characterized by X-ray diffraction, field emission scanning electron microscopy, and energy dispersive X-ray spectroscopy. Results showed that the ChOx/CeO2 NPs/PPy/electrode was linearly related with cholesterol in the range of 50 to 500 mg/dL. The sensitivity of ChOx/CeO2 NPs/PPy/electrode was 5.7 × 10 − 6  mA/mg⋅dL−1. The optimal parameters, including pH value and temperature, and selectivity, storage stability, and reproducibility of ChOx/CeO2 NPs/PPy/electrode were investigated.

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