Transplantation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Improves Cartilage Repair in a Rabbit Model | Zendy

Guihua Yang | Zendy; Jiashen Shao | Zendy; Jiachen Lin | Zendy; Haixia Yang | Zendy; Jing Jin | Zendy; Chenxi Yu | Zendy; Bo Shen | Zendy; Xiaorui Hu | Zendy; Huijie Si | Zendy; Xiaoxin Li | Zendy; Yuchen Niu | Zendy; Zhihong Wu | Zendy

Open Access

Transplantation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Improves Cartilage Repair in a Rabbit Model

Author(s) -

Guihua Yang,

Jiashen Shao,

Jiachen Lin,

Haixia Yang,

Jing Jin,

Chenxi Yu,

Bo Shen,

Xiaorui Hu,

Huijie Si,

Xiaoxin Li,

Yuchen Niu,

Zhihong Wu

Publication year - 2021

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2021/6380141

Subject(s) - mesenchymal stem cell , cd90 , cartilage , transplantation , umbilical cord , medicine , stem cell transplantation for articular cartilage repair , stem cell , pathology , regeneration (biology) , wharton's jelly , cd34 , bone marrow , immunology , adult stem cell , biology , anatomy , cellular differentiation , surgery , microbiology and biotechnology , gene , biochemistry

The aim of this study was to investigate the therapeutic efficacy and safety of transplanting human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) in the treatment of cartilage injury. First, the articular cartilage defect model in rabbits was constructed. Then, the identified hUCB-MSCs and rabbit bone marrow stem cells (rBM-MSCs) were transplanted into the bone defect, respectively, and the cartilage repair effect was observed by hematoxylin-eosin (HE) staining and immunohistochemistry. Besides, the glycosaminoglycan (GAG) content and biomechanics of the restoration area were also evaluated. In our study, hUCB-MSCs and rBM-MSCs exhibited typical MSC characteristics, with positive expressions of CD73, CD105, and CD90 and negative for CD45, CD34, CD14, and HLA-DR. After the transplantation of hUCB-MSCs and rBM-MSCs, the overall quality of cartilage tissue was significantly improved, and the recipients did not show significant side effects in general. However, the expression of matrix metalloproteinase-13 (MMP-13) in the de novo tissues of the hUCB-MSCs and rBM-MSCs groups was both increased, indicating that the novel tissues may have some potential osteoarthritic changes. In conclusion, our results suggest the therapeutic effect of hUCB-MSCs transplantation in cartilage regeneration, providing a promising future in the clinical treatment of cartilage injury.

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