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Background    Sedum dendroideum  has antioxidant effects that are beneficial for different diseases. We aimed to analyze the antiproliferative activity of  S. dendroideum  in human pterygium fibroblasts (HPFs).Methods   HPFs were treated for 24 h with 0–1000  μ g/mL of  S. dendroideum  lyophilized to analyze its effect on cell viability using the CellTiter assay. RNA from HPF treated with 250  μ g/mL of  S. dendroideum  lyophilized was isolated, and the expression of VEGF and CTGF genes was evaluated by qPCR. A dermal fibroblast cell line (HDFa) was used as a healthy control. The total phenolic content, antioxidant activity, and chemical profile of  S. dendroideum  lyophilized were determined.Results   Viability of HPF decreased after 24 h treatment of  S. dendroideum  in a dose-dependent manner. The expression of VEGF and CTGF significantly decreased ( P  < 0.01) in HPF treated with 250  μ g/mL of  S. dendroideum  when compared with untreated HPF. The total phenolic concentration in the  S. dendroideum  lyophilized was 33.67 mg gallic acid equivalents (GAE)/g. Antioxidant activity was 384.49 mM Trolox equivalents/mL. The main phenolic compounds identified by HPLC analysis were the kaempferol-3-O-glycoside, kaempferol-3-O-rhamnoside, kaempferol-3-O-neohesperidoside-7-O- α -rhamnopyranoside, and kaempferol-3-O-glycoside-7-O-rhamnoside.Conclusions    S. dendroideum  decreases the proliferation of HPF and the expression of VEGF and CTGF. The phenolic compound concentration, antioxidant activity, and phytochemical profile may play a role in these effects.

Phytochemical Profile and Antioxidant and Antiproliferative Activity of Sedum dendroideum on Pterygium Fibroblasts