Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation | Zendy

Elize Wolmarans | Zendy; Juanita Mellet | Zendy; Chrisna Durandt | Zendy; Fourie Joubert | Zendy; Michael S. Pepper | Zendy

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Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation

Author(s) -

Elize Wolmarans,

Juanita Mellet,

Chrisna Durandt,

Fourie Joubert,

Michael S. Pepper

Publication year - 2021

Publication title -

stem cells international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.205

H-Index - 64

eISSN - 1687-9678

pISSN - 1687-966X

DOI - 10.1155/2021/5595172

Subject(s) - transcriptome , stromal cell , adipose tissue , cell , population , microbiology and biotechnology , biology , mesenchymal stem cell , vascular smooth muscle , gene expression , gene , smooth muscle , cancer research , medicine , genetics , endocrinology , environmental health

The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs that is not well understood is their heterogeneity. The aim of this exploratory study was to characterize the heterogeneity of freshly isolated hASCs after two population doublings (P2) using single-cell transcriptome analysis. A minimum of two subpopulations were identified at P2. A major subpopulation was identified as contractile cells which, based on gene expression patterns, are likely to be pericytes and/or vascular smooth muscle cells (vSMCs).

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