Preparation and Characterization of Polyamidoamine G2.0-Hematin as a Biocatalyst for Fabricating Catecholic Gelatin Hydrogel | Zendy

Tan Phuoc Ton | Zendy; Trần Thị Yến Nhi | Zendy; Le Hang Dang | Zendy; Kim Thi Hoang Nguyen | Zendy; Đoàn Đình Phương | Zendy; Van Toan Nguyen | Zendy; Dinh Trung Nguyen | Zendy; Bao Huy Le | Zendy; Ngoc Quyên Trân | Zendy; Bich Tram Nguyen | Zendy

Open Access

Preparation and Characterization of Polyamidoamine G2.0-Hematin as a Biocatalyst for Fabricating Catecholic Gelatin Hydrogel

Author(s) -

Tan Phuoc Ton,

Trần Thị Yến Nhi,

Le Hang Dang,

Kim Thi Hoang Nguyen,

Đoàn Đình Phương,

Van Toan Nguyen,

Dinh Trung Nguyen,

Bao Huy Le,

Ngoc Quyên Trân,

Bich Tram Nguyen

Publication year - 2021

Publication title -

international journal of polymer science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.399

H-Index - 33

eISSN - 1687-9430

pISSN - 1687-9422

DOI - 10.1155/2021/5563229

Subject(s) - gelatin , self healing hydrogels , horseradish peroxidase , zeta potential , biocatalysis , click chemistry , nuclear chemistry , materials science , chemistry , enzyme , polymer chemistry , nanotechnology , biochemistry , catalysis , nanoparticle , ionic liquid

In this study, we report that an enzyme-mimicking biocatalyst polyamidoamine (PAMAM) dendrimer G2.0-hematin (G2.0-He) was fabricated successfully. The chemical structure of G2.0-He was verified by 1H NMR and FT-IR spectroscopy. G2.0-He exhibited a size distribution from 11.6 ± 1.7 nm to 12.5 ± 2.9 nm and a zeta potential from 32.5 mV to 25.6 mV along with the enhancement of the hematin conjugation degree. The relative activity of G2.0-He was evaluated based on pyrogallol oxidation reactions at pH = 7 . The results showed that G2.0-He was more stable than horseradish peroxidase (HRP) enzyme in high H2O2 concentrations. The HRP-mimic ability of G2.0-He was also confirmed by the catalyzation when preparing catecholic gelatin hydrogels under mild conditions. Moreover, our results also revealed that these hydrogels performed with excellent cytocompatibility in an in vitro study and could be used as a potential scaffold for adhesion and proliferation of fibroblast cells. The obtained results indicated that G2.0-He is a suitable platform for altering the HRP enzyme in several biomedical applications.

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