lncRNA MAGI2-AS3 Exerts Antioncogenic Roles in Hepatocellular Carcinoma via Regulating the miR-519c-3p/TXNIP Axis | Zendy

Huamei Wei | Zendy; Qianli Tang | Zendy; Anmin Wang | Zendy; Ya Zhang | Zendy; Zebang Qin | Zendy; Wenchuan Li | Zendy; Zuoming Xu | Zendy; Jianchu Wang | Zendy; Jian Pu | Zendy

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lncRNA MAGI2-AS3 Exerts Antioncogenic Roles in Hepatocellular Carcinoma via Regulating the miR-519c-3p/TXNIP Axis

Author(s) -

Huamei Wei,

Qianli Tang,

Anmin Wang,

Ya Zhang,

Zebang Qin,

Wenchuan Li,

Zuoming Xu,

Jianchu Wang,

Jian Pu

Publication year - 2021

Publication title -

journal of oncology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.228

H-Index - 54

eISSN - 1687-8469

pISSN - 1687-8450

DOI - 10.1155/2021/5547345

Subject(s) - txnip , gene knockdown , cancer research , cell growth , carcinogenesis , hepatocellular carcinoma , medicine , in vivo , competing endogenous rna , thioredoxin interacting protein , flow cytometry , small hairpin rna , cell , microbiology and biotechnology , downregulation and upregulation , long non coding rna , apoptosis , biology , cancer , gene , thioredoxin , biochemistry , oxidative stress

Our work was aimed to explore the mechanisms of MAGI2 antisense RNA 3 (MAGI2-AS3) in regulating hepatocellular carcinoma (HCC) carcinogenesis.Methods MAGI2-AS3, microRNA-519c-3p (miR-519c-3p), and thioredoxin interacting protein (TXNIP) levels in HCC were detected by the RT-qPCR method. Cell proliferation and apoptosis rate were measured using Cell Counting Kit-8 assay and flow cytometry assay. Relationship between MAGI2-AS3, TXNIP, and miR-519c-3p were analyzed via luciferase activity assay, RNA pull-down assay, and RNA immunoprecipitation assay. Mouse xenograft models of HCC were conducted to explore the roles of MAGI2-AS3 in vivo.Results MAGI2-AS3 levels were elevated, and miR-519c-3p decreased in HCC. MAGI2-AS3 overexpression inhibits while its knockdown stimulates HCC cell growth through miR-519c-3p. Moreover, miR-519c-3p overexpression stimulates HCC cell growth. MAGI2-AS3 serves as competing endogenous RNA (ceRNA) of miR-519c-3p to regulate TXNIP in HCC. And, TXNIP upregulation weakened the influence of MAGI2-AS3 knockdown on HCC cell behaviors. Additionally, MAGI2-AS3 overexpression suppressed HCC tumor growth in vivo.Conclusion MAGI2-AS3 inhibits HCC tumorigenesis through miR-519c-3p/TXNIP axis in vitro and in vivo, indicating MAGI2-AS3 plays a crucial role in HCC development.

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