Antiangiogenic Effect of Platelet P2Y12 Inhibitor in Ischemia-Induced Angiogenesis in Mice Hindlimb | Zendy

Xiaoli Wang | Zendy; Huan Zhao | Zendy; Naiquan Yang | Zendy; Yue Jin | Zendy; Jianguo Chen | Zendy

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Antiangiogenic Effect of Platelet P2Y12 Inhibitor in Ischemia-Induced Angiogenesis in Mice Hindlimb

Author(s) -

Xiaoli Wang,

Huan Zhao,

Naiquan Yang,

Yue Jin,

Jianguo Chen

Publication year - 2021

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2021/5529431

Subject(s) - angiogenesis , hindlimb , cd31 , ticagrelor , western blot , inflammation , ischemia , medicine , perfusion , vascular endothelial growth factor , endocrinology , p2y12 , therapeutic angiogenesis , platelet , pharmacology , neovascularization , chemistry , vegf receptors , biochemistry , aspirin , platelet aggregation , gene , clopidogrel

Purpose Postischemic inflammation induces angiogenesis, while platelet P2Y 12 inhibitors can alleviate this inflammation. Therefore, we studied the potential effects of P2Y 12 inhibitor, ticagrelor, on angiogenesis in a mouse model of hindlimb ischemia.Methods Laser Doppler perfusion imaging and capillary density measurement were used for angiogenesis quantified. Immunofluorescence was used to detect the level of CD31. The mice muscle was harvested for enzyme-linked immunosorbent (ELISA) assay of interleukin- (IL) 10 activity and Western blot determination of vascular endothelial growth factor (VEGF) production.Results Ischemic hindlimb angiogenesis was sharply decreased in IL-10 +/+ mice than IL-10 −/− mice. Ticagrelor inhibited angiogenesis and blood reperfusion recovery significantly elevated the levels of IL-10 and decreased the expression of VEGF in the IL-10 +/+ mouse ischemic hindlimb, which were abolished in IL-10-deficient (IL-10 –/– ) C57BL/6J mice.Conclusion The study underscores that the effect of ticagrelor antiangiogenic function is related with the higher IL-10 expression.

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