Treadmill Exercise during Pregnancy Decreased Vulnerability to Neonatal Hypoxia-Ischemia through Reducing Inflammation and Increasing Antiapoptotic Gene Expressions and Antioxidant Capacity in Rats | Zendy

Elahe Gorgij | Zendy; Hamed Fanaei | Zendy; Parichehr Yaghmaei | Zendy; Mohammad Reza Shahraki | Zendy; Hadi Mirahmadi | Zendy

Open Access

Treadmill Exercise during Pregnancy Decreased Vulnerability to Neonatal Hypoxia-Ischemia through Reducing Inflammation and Increasing Antiapoptotic Gene Expressions and Antioxidant Capacity in Rats

Author(s) -

Elahe Gorgij,

Hamed Fanaei,

Parichehr Yaghmaei,

Mohammad Reza Shahraki,

Hadi Mirahmadi

Publication year - 2021

Publication title -

stroke research and treatment

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.939

H-Index - 34

eISSN - 2090-8105

pISSN - 2042-0056

DOI - 10.1155/2021/5512745

Subject(s) - medicine , hypoxia (environmental) , inflammation , antioxidant , pregnancy , treadmill , antioxidant capacity , ischemia , bioinformatics , oxidative stress , physical therapy , physiology , genetics , biology , chemistry , organic chemistry , oxygen , biochemistry

Background The purpose of present study was to assess the impact of maternal treadmill exercise during pregnancy on inflammation, oxidative stress, expression of Bax and Bcl-2 genes, and brain-derived neurotrophic factor (BDNF) level in neonatal rat brain after the hypoxia-ischemia injury. Material and Methods . A total of 24 female Wistar rats were utilized in this research. Two groups are randomly considered for rats: (1) not exercised through pregnancy and (2) exercised during pregnancy. Offsprings were divided into four groups including after delivery: (1) sham, (2) sham/exercise (sham/EX), (3) HI, and (4) HI+exercise. HI was induced in pups at postnatal day 8. Neurobehavioral tests were done seven days after HI induction. Then, the brain tissue was taken from the skull to estimate Bcl-2 and Bax gene expressions, BDNF, cerebral edema, infarct volume, inflammatory factors, oxidative stress, and neurological function.Results The BDNF level in the HI+exercise group was considerably higher than the HI, sham, and sham/EX groups. Tumor necrosis factor (TNF- α ), C-reactive protein (CRP), and the whole oxidant capacity (TOC) levels in the HI group were significantly higher than the sham and sham/EX groups. TNF- α , CRP, and TOC levels in the HI+exercise group were significantly lower than the HI group. Total antioxidant capacity (TAC) level in the HI+exercise group was significantly higher than the HI group. Infarct volume and edema percent in the HI+exercise group were significantly lower than the HI group. Neurological function in the HI+exercise group was significantly better than the HI group. Bax expression in the HI+exercise group was significantly lower than the HI group. Bcl-2 expression in the HI+exercise group was significantly higher than the HI group. In the sham group, BDNF, TNF- α , CRP, TAC, TOC, edema levels, and neurological function had no significant difference with the sham/EX group.Conclusion It appears that the maternal treadmill exercise during pregnancy exerts a supportive impact against neonatal HI brain injury through increasing antioxidant capacity, Bcl-2 expression, and BDNF levels and decreasing inflammation that is resulted in the lower infarct volume and sensorimotor dysfunction.

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