Functionalized Ferroferric Oxide Nanomagnetic Beads for Extraction of Nucleic Acid and Its Application in Early Screening of Colorectal Cancer | Zendy

Bing Pei | Zendy; Zhenjiang Zhang | Zendy; Jian Sun | Zendy; Xiaoyan Qi | Zendy; Qian Cui | Zendy; You Yan | Zendy; xiaoYan Wang | Zendy; Miaomiao Yang | Zendy; Chunjie Song | Zendy; Lingsi Yin | Zendy; Juan Wu | Zendy; Zhixin Geng | Zendy; Yue Bang Wang | Zendy; Yi Lü | Zendy

Open Access

Functionalized Ferroferric Oxide Nanomagnetic Beads for Extraction of Nucleic Acid and Its Application in Early Screening of Colorectal Cancer

Author(s) -

Bing Pei,

Zhenjiang Zhang,

Jian Sun,

Xiaoyan Qi,

Qian Cui,

You Yan,

xiaoYan Wang,

Miaomiao Yang,

Chunjie Song,

Lingsi Yin,

Juan Wu,

Zhixin Geng,

Yue Bang Wang,

Yi Lü

Publication year - 2021

Publication title -

journal of healthcare engineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 29

eISSN - 2040-2309

pISSN - 2040-2295

DOI - 10.1155/2021/5230666

Subject(s) - nucleic acid , colorectal cancer , magnetic bead , dna extraction , chromatography , polymerase chain reaction , dna , extraction (chemistry) , feces , cancer , chemistry , biology , biochemistry , microbiology and biotechnology , genetics , gene

Early screening is an important way to reduce the incidence and mortality of colorectal cancer (CRC). Fecal DNA testing stands out among many screening methods due to its high sensitivity. However, at this stage, researchers have not found a high-efficiency method for fecal DNA extraction. To this end, this work carried out a new round of exploration. Here, this experiment synthesized a kind of nanomagnetic beads (NH2-SiO2@Fe3O4) with good stability for nucleic acid extraction. A comparative study with the centrifugal adsorption column method revealed the significant advantages of the magnetic bead method in extracting fecal DNA. The DNA extracted by the magnetic bead method is of high purity, can also achieve high-throughput tests, and is more suitable for polymerase chain reaction detection, greatly simplifying the stool DNA detection process and providing a basis for the widespread promotion of early screening.

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