Open AccessCarnosic Acid Induces Antiproliferation and Anti-Metastatic Property of Esophageal Cancer Cells via MAPK Signaling Pathways
Author(s) -
SiCong Jiang,
Yinda Qiu,
Zhaozhen Wang,
Yulong Ji,
Xiaofang Zhang,
Xia Yan,
Zhiqiang Zhan
Publication year - 2021
Publication title -
journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.228
H-Index - 54
eISSN - 1687-8469
pISSN - 1687-8450
DOI - 10.1155/2021/4451533
Subject(s) - apoptosis , cancer research , cell cycle , cell growth , flow cytometry , mapk/erk pathway , carnosic acid , signal transduction , cell , mtt assay , medicine , chemistry , microbiology and biotechnology , biology , immunology , biochemistry , antioxidant
Background Carnosic acid (CA) is a polyphenolic diterpene extracted from rosemary. Reports have shown that CA possesses anticancer activity. However, whether CA inhibits esophageal squamous cell carcinoma, an aggressive type of esophageal cancer, remains untested.Methods The effects of CA on cell survival, migration, and apoptosis were evaluated by a combination of MTT, colony formation assay, flow cytometry, and Transwell assay. The potential signaling pathways involved were investigated via Western blot assay.Results CA dose-dependently inhibited cell proliferation, apoptosis, migration, and colony formation. Mechanistically, CA arrested the cell cycle at G2/M phase, promoted cell apoptosis, induced DNA damage, and inhibited the MAPK signaling pathways.Conclusion Our results suggest that CA is a potential anticancer drug for esophageal squamous cell carcinoma.