Open AccessmiR-190a-3p Promotes Proliferation and Migration in Glioma Cells via YOD1
Author(s) -
Lili Zhou,
Lingzhi Li,
Yan Chen,
Chun Chen,
Zhongwen Zhi,
Luxia Yan,
Yuqian Wang,
Bing Liu,
Qijin Zhai
Publication year - 2021
Publication title -
computational and mathematical methods in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.462
H-Index - 48
eISSN - 1748-6718
pISSN - 1748-670X
DOI - 10.1155/2021/3957738
Subject(s) - glioma , luciferase , reporter gene , cell culture , cancer research , cell growth , biology , function (biology) , microrna , microbiology and biotechnology , gene , transfection , gene expression , genetics
To investigate the function of miR-190a-3p on the proliferation and migration of glioma.Methods Twenty glioma samples and 6 normal brain tissue samples were collected. Normal human glial cell line HEB and glioma cell lines were used for the experiments. We then used TargetScan to predict the target genes of miR-190a-3p. Dual-luciferase reporter assay was also used to validate.Results Combined with dual-luciferase reporter experiment, we finally verified that YOD1 was the aim, and it was low-expressed in glioma. Besides, a series of mechanism experiments then proved that miR-190a-3p negatively regulates YOD1 expression.Conclusions Our research was the first to demonstrate the promoting function of miR-190a-3p in the proliferation and migration of glioma and provided new views for the treatment of glioma. miR-190a-3p was expected to be a new target for molecular therapy of glioma.
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