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The present study evaluated first the characterization of  Teff  straw and then Box–Behnken design (BBD), and response surface methodology was adopted to optimize the parameters (hydrolysis temperature, dilute sulfuric acid concentration, solid to liquid ratio, and hydrolysis time) of dilute sulfuric acid hydrolysis of  Teff  straw in order to get a maximum yield of total reducing sugar (TRS). The chemical analysis of  Teff  straw revealed high amounts of cellulose (41.8 wt%), hemicellulose (38 wt%), and lignin (17 wt%). The morphological analysis using SEM showed that hydrolyzed  Teff  straw with dilute sulfuric acid has more pores and distorted bundles than those of raw  Teff  straw. XRD analysis also indicated that hydrolyzed  Teff  straw has higher crystallinity index and smaller crystallite size than raw  Teff  straw, which might be due to removal of hemicellulose, amorphous cellulose, and lignin components. Under the optimized conditions for dilute sulfuric acid hydrolysis of  Teff  straw (120°C, 4% v/v H 2 SO 4  concentration, 1 : 20 solid to liquid ratio, and 55 min hydrolysis time), we have found a total reducing sugar yield of 26.65 mg/g. The results of validation experiment under the optimum conditions agreed well with model predictions.

international journal of biomaterials

Reducing Sugar Production from Teff Straw Biomass Using Dilute Sulfuric Acid Hydrolysis: Characterization and Optimization Using Response Surface Methodology