Anti-Inflammatory Effects of Lagerstroemia ovalifolia Teijsm. & Binn. in TNFα/IFNγ-Stimulated Keratinocytes | Zendy

Han-Sol Lee | Zendy; JinHyub Paik | Zendy; OkKyoung Kwon | Zendy; Imam Paryanto | Zendy; Prasetyawan Yuniato | Zendy; Hyung Won Ryu | Zendy; Sangho Choi | Zendy; SeiRyang Oh | Zendy; SangBae Han | Zendy; JiWon Park | Zendy; KyungSeop Ahn | Zendy

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Anti-Inflammatory Effects of Lagerstroemia ovalifolia Teijsm. & Binn. in TNFα/IFNγ-Stimulated Keratinocytes

Author(s) -

Han-Sol Lee,

JinHyub Paik,

OkKyoung Kwon,

Imam Paryanto,

Prasetyawan Yuniato,

Hyung Won Ryu,

Sangho Choi,

SeiRyang Oh,

SangBae Han,

JiWon Park,

KyungSeop Ahn

Publication year - 2021

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2021/2439231

Subject(s) - hacat , proinflammatory cytokine , chemokine , ccl22 , immunology , ccl17 , protein kinase b , tumor necrosis factor alpha , pi3k/akt/mtor pathway , cancer research , chemistry , medicine , cxcl10 , inflammation , phosphorylation , signal transduction , biochemistry , in vitro

Ethnopharmacological Relevance . Atopic dermatitis is a chronic inflammatory skin disease. Lagerstroemia ovalifolia Teijsm. & Binn. (LO) has traditionally been used as an herbal medicine for anti-inflammatory diseases. The effect of LO on atopic dermatitis has not been verified scientifically. We investigated the effects of CHCl 3 fraction number 5 of LO (LOC) on atopic dermatitis through cell-based experiments. HaCaT cells were treated with tumor necrosis factor-alpha (TNF α )/interferon-gamma (IFN γ ) to induce an inflammatory reaction. Proinflammatory cytokines, interleukin- (IL-) 6, IL-8, and IL-1 β and chemokines such as thymus and activation-regulated chemokine (TARC/CCL17), monocyte chemoattractant protein 1 (MCP1/CCL2), and macrophage-derived chemokine (MDC/CCL22) were measured by RT-PCR and ELISA. In addition, the degree of phosphorylation and activation of JAK/STAT1, PI3K/AKT, and nuclear factor-kappa B (NF- κ B) were measured by western blot and luciferase assays. The production of inflammatory cytokines and chemokines and activation of the JAK/STAT1, PI3K/AKT, and NF- κ B pathways were induced by TNF α /IFN γ in HaCaT cells. Under these conditions, LOC treatment inhibited the production of targeted cytokines and chemokines and decreased the phosphorylation and activation of JAK/STAT1, PI3K/AKT, and NF- κ B. These results suggest that LOC reduces the production of proinflammatory cytokines and chemokines by suppressing the JAK/STAT1, PI3K/AKT, and NF- κ B pathways. Therefore, LOC may have potential as a drug for atopic dermatitis.

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