Open AccessEpigenetic Silencing of SOX15 Is Controlled by miRNAs rather than Methylation in Papillary Thyroid Cancer
Author(s) -
Ahmet Özaydın,
A.O. Soysal,
Betül Seyhan,
Soykan Arıkan,
Nejat Dalay,
Nur Buyru
Publication year - 2021
Publication title -
disease markers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 66
eISSN - 1875-8630
pISSN - 0278-0240
DOI - 10.1155/2021/1588220
Subject(s) - methylation , epigenetics , microrna , gene silencing , biology , dna methylation , thyroid carcinoma , cancer research , cancer , thyroid cancer , papillary thyroid cancer , pathogenesis , gene expression , pathology , thyroid , microbiology and biotechnology , gene , medicine , genetics , immunology
Methods In this study, qRT-PCR was used to investigate the expression levels of the SOX15 gene and of miR-182, miR-183, miR-375, and miR-96 in thyroid tumors and adjacent noncancerous tissues. We also investigated the methylation status of the SOX15 promoter by methylation-specific PCR in tumors and adjacent noncancerous tissues.Results We observed a statistically significant downregulation of SOX15 expression in tumors compared to noncancerous tissue samples. The methylation levels of tumors and matched noncancerous tissues were similar, but miR-182, miR-183, and miR-375 expression levels were elevated in tumor tissues compared to noncancerous tissue samples.Conclusions Our results indicate that SOX15 gene expression is associated with the pathogenesis of papillary thyroid carcinoma (PTC), and the epigenetic control of the SOX15 gene is regulated by miRNAs rather than by promoter methylation.
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