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Spirosoma linguale  is a free-living nonpathogenic organism. Like many other bacteria,  S. linguale  produces a cell-associated  α -N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of  α -N-acetylgalactosamine at pH 7. Calculated K M  was 1.1 mM with k cat  of 173 s −1 . The described enzyme belongs to the GH109 family.

enzyme research

Enzymatic Conversion of RBCs by <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>α</mml:mi></mml:mrow></mml:math>-N-Acetylgalactosaminidase from<i> Spirosoma linguale</i>

Enzymatic Conversion of RBCs by α-N-Acetylgalactosaminidase from Spirosoma linguale