High Expression of Galectin-3 in Patients with IgG4-Related Disease: A Proteomic Approach | Zendy

Adeeb Salah | Zendy; Hajime Yoshifuji | Zendy; Shinji Ito | Zendy; Koji Kitagori | Zendy; Kaori Kiso | Zendy; Norishige Yamada | Zendy; Toshiki Nakajima | Zendy; Hironori Haga | Zendy; Tatsuaki Tsuruyama | Zendy; Aya MiyagawaHayashino | Zendy

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High Expression of Galectin-3 in Patients with IgG4-Related Disease: A Proteomic Approach

Author(s) -

Adeeb Salah,

Hajime Yoshifuji,

Shinji Ito,

Koji Kitagori,

Kaori Kiso,

Norishige Yamada,

Toshiki Nakajima,

Hironori Haga,

Tatsuaki Tsuruyama,

Aya MiyagawaHayashino

Publication year - 2017

Publication title -

pathology research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.398

H-Index - 21

eISSN - 2090-8091

pISSN - 2042-003X

DOI - 10.1155/2017/9312142

Subject(s) - medicine , galectin , galectin 3 , pathology , disease , protein expression , expression (computer science) , igg4 related disease , computational biology , bioinformatics , immunology , genetics , biology , gene , computer science , programming language

Objectives Immunoglobulin G4-related disease (IgG4-RD) is a multiorgan condition manifesting itself in different forms. This study aimed to investigate protein expression profiles and to find the possible biomarker for IgG4-RD by liquid chromatography mass spectrometry (LC-MS) using tissue sections in IgG4-RD patients.Methods Protein expression profiles in five IgG4-related pancreatitis and three normal pancreatic samples were compared using LC-MS and were validated by quantitative real-time PCR (qRT-PCR), immunoblotting, and immunohistochemistry. ELISA was employed in the serum of 20 patients with systemic IgG4-RD before and during steroid treatment.Results LC-MS indicated that the levels of 17 proteins were significantly higher and 12 others were significantly lower in IgG4-related pancreatitis patients compared to controls. Among these proteins, galectin-3 levels were 13-fold higher in IgG4-related pancreatitis ( P < 0.01). These results were confirmed by immunoblotting and qRT-PCR. The average number of galectin-3 + cells in various organs of IgG4-RD patients, including salivary glands, lungs, and lymph nodes, was higher than in controls. Galectin-3 was detectable in macrophages, dendritic cells, and stromal myofibroblast-like cells, but not in lymphocytes by immunofluorescence staining. Serum galectin-3 levels were higher in patients with IgG4-RD compared with healthy donors and remained high during steroid therapy.Conclusion Galectin-3 was overexpressed in IgG4-RD and the levels were indirectly related to clinical activity.

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