Molecular Identification and Traceability of Illegal Trading inLignobrycon myersi(Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode | Zendy

Alexandre dos SantosRodrigues | Zendy; José Henrique Souza Galdino Brandão | Zendy; Jamille de Araújo Bitencourt | Zendy; Ricardo JucáChagas | Zendy; Iracilda Sampaio | Zendy; Horácio Schneider | Zendy; Paulo Roberto Antunes de Mello Affonso | Zendy

Open Access

Molecular Identification and Traceability of Illegal Trading inLignobrycon myersi(Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode

Author(s) -

Alexandre dos SantosRodrigues,

José Henrique Souza Galdino Brandão,

Jamille de Araújo Bitencourt,

Ricardo JucáChagas,

Iracilda Sampaio,

Horácio Schneider,

Paulo Roberto Antunes de Mello Affonso

Publication year - 2016

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1155/2016/9382613

Subject(s) - threatened species , dna barcoding , biology , population , cytochrome c oxidase subunit i , freshwater fish , characiformes , fishery , zoology , mitochondrial dna , ecology , fish <actinopterygii> , genetics , demography , sociology , habitat , gene

Lignobrycon myersi is a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species, L. myersi is occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset for L. myersi , we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species ( Triportheus spp.) and other fish species ( Astyanax fasciatus ) commonly mixed with L. myersi in street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminating L. myersi specimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading of L. myersi and to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.

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