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Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) production using new alkaliphile  Microbacterium terrae  KNR 9 was investigated by submerged fermentation. Statistical screening for components belonging to different categories, namely, soluble and raw starches as carbon sources, complex organic and inorganic nitrogen sources, minerals, a buffering agent, and a surfactant, has been carried out for CGTase production using Plackett-Burman factorial design. To screen out  k  (19), number of variables,  k  + 1 (20), number of experiments, were performed. Among the fourteen components screened, four components, namely, soluble starch, corn flour, yeast extract, and K 2 HPO 4 , were identified as significant with reference to their concentration effect and corresponding  p  value. Although soluble starch showed highest significance, comparable significance was also observed with corn flour and hence it was selected as a sole carbon source along with yeast extract and K 2 HPO 4  for further media optimization studies. Using screened components, CGTase production was increased to 45% and 87% at shake flask level and laboratory scale fermenter, respectively, as compared to basal media.

biotechnology research international

Screening and Selection of Medium Components for Cyclodextrin Glucanotransferase Production by New Alkaliphile<i> Microbacterium terrae</i> KNR 9 Using Plackett-Burman Design

Screening and Selection of Medium Components for Cyclodextrin Glucanotransferase Production by New Alkaliphile Microbacterium terrae KNR 9 Using Plackett-Burman Design