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Fungicide was an important part in mycopesticides, which play an important role in pest management, while their mass production and commercialization faced problem. We found that the nutrition for mycelia growth and sporulation differences a lot. So, we developed “two-step method” to define the nutrition for sporulation in this paper. The results indicated that the novel method led to a great increase of spore yields for  Beauveria bassiana  (IBC1201),  Lecanicillium lecanii  (CA-1-G), and  Pochonia chlamydosporia  (HSY-12-14), respectively, of about 100, 2, and 16 times and, also reduced the cycle of mass production to 1/3 compared with common time for culturing.

Application of Two-Stage Cultivation for Exploring the Nutritional Requirements for Sporulation of Three Biocontrol Fungi