Effect of Moderate UVC Irradiation on Bovine Serum Albumin and Complex with Antimetabolite 5-Fluorouracil: Fluorescence Spectroscopic and Molecular Modelling Studies | Zendy

Shanmugavel Chinnathambi | Zendy; S. Karthikeyan | Zendy; D. Velmurugan | Zendy; Nobutaka Hanagata | Zendy; Prakasarao Aruna | Zendy; Singaravelu Ganesan | Zendy

Open Access

Effect of Moderate UVC Irradiation on Bovine Serum Albumin and Complex with Antimetabolite 5-Fluorouracil: Fluorescence Spectroscopic and Molecular Modelling Studies

Author(s) -

Shanmugavel Chinnathambi,

S. Karthikeyan,

D. Velmurugan,

Nobutaka Hanagata,

Prakasarao Aruna,

Singaravelu Ganesan

Publication year - 2015

Publication title -

international journal of spectroscopy

Language(s) - English

Resource type - Journals

eISSN - 1687-9457

pISSN - 1687-9449

DOI - 10.1155/2015/315764

Subject(s) - antimetabolite , bovine serum albumin , chemistry , fluorescence , fluorescence spectroscopy , irradiation , serum albumin , biophysics , chromatography , photochemistry , nuclear chemistry , biochemistry , toxicity , biology , organic chemistry , physics , quantum mechanics , nuclear physics

The interaction of antimetabolite 5-fluorouracil (5FU) with bovine serum albumin (BSA) under UVC (253.7 nm) irradiation was investigated in the present study using UV-Vis spectroscopy, steady state/time resolved fluorescence spectroscopic techniques. The stability of protein was found to be very strong when BSA gets bind to 5FU and moreover it is compared with the free BSA under UVC irradiation. From the fluorescence spectroscopic study, the stability of the complex was found to acquire 2-fold stronger than free protein. From the molecular modelling studies, we came to know the hydrogen bonds between BSA and antimetabolite 5FU are strong, up to 70.4 J/m2 under UVC irradiation

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