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Artemisia absinthium is an important medicinal plant. Owing to the increasing anthropogenic activities and demand from the pharmaceutical industry, this plant species is overexploited; thereby this endangered its genetic stock in the wild. Therefore, it is urgently needed to develop nonconventional methods for conservation of A. absinthium. Nodal segments obtained from the field grown 2-month-old plants were used as explants. Murashige and Skoog (MS) medium containing 0.5 mg/L 6-benzylaminopurine (BAP) and 0.25 mg/L kinetin (Kn) were reported to be optimum for induction of shoots (6.0 ± 0.52 shoots per explant). The shoots were multiplied by repeated transfer of original explants and by subculturing of in vitro raised shoots on MS medium augmented with 1.0 mg/L each of BAP and Kn and 0.1 mg/L α-naphthaleneacetic acid (NAA). All in vitro regenerated shoots (100%) were rooted (4.4 ± 0.35 roots) on one-fourth strength MS medium supplemented with 2.0 mg/L indole-3 butyric acid (IBA). Cent percentage shoots rooted ex vitro on sterile Soilrite under the greenhouse conditions when the shoots were treated with 200 mg/L of IBA for 5 min. Plantlets rooted in vitro and ex vitro were acclimatized successfully in the greenhouse and exhibited 87% and 95% survival rate

EfficientIn VitroPropagation byEx VitroRooting Methods ofArtemisia absinthiumL., an Ethnobotanically Important Plant