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A Transcriptome Post-Scaffolding Method for Assembling High Quality Contigs
Author(s) -
Mingming Liu,
Zach N. Adelman,
Kevin M. Myles,
Liqing Zhang
Publication year - 2014
Publication title -
computational biology journal
Language(s) - English
Resource type - Journals
eISSN - 2314-4173
pISSN - 2314-4165
DOI - 10.1155/2014/961823
Subject(s) - transcriptome , contig , sequence assembly , computational biology , redundancy (engineering) , biology , computer science , de novo transcriptome assembly , genome , genetics , gene , gene expression , operating system
As the development of the high throughput sequencing technologies, transcriptome can be sequenced with a low price and high efficiency. Sequence assembly approaches have been renewed to meet the new requirements from new sequencing technologies. Assembly strategies are important for biologists who need to assemble the transcriptome generated in their experiments. However, some modern de novo assembly strategies generate a large section of redundant contigs due to sequence variations, which greatly affect downstream analysis and experiments. This work proposed TransPS, a post transcriptome scaffolding method to generate high quality transcriptomes.

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