Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor | Zendy

Masoumeh Fakhr Taha | Zendy; Arash Javeri | Zendy; Sara Rohban | Zendy; Seyed Javad Mowla | Zendy

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Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor

Author(s) -

Masoumeh Fakhr Taha,

Arash Javeri,

Sara Rohban,

Seyed Javad Mowla

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/941486

Subject(s) - homeobox protein nanog , sox2 , rex1 , stem cell , mesenchymal stem cell , leukemia inhibitory factor , biology , adipose tissue , microbiology and biotechnology , embryonic stem cell , adult stem cell , induced pluripotent stem cell , genetics , endocrinology , gene

The expression pattern of pluripotency markers in adipose tissue-derived stem cells (ADSCs) is a subject of controversy. Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs. In the present study, we studied the roles of leukemia inhibitory factor (LIF) and miR-302 in this regard. Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and pluripotency markers. One day after plating, ADSCs expressed OCT4 and Sox2 proteins. After three passages, the expression of hematopoietic and pluripotency markers decreased, while the expression of mesenchymal stem cell markers exhibited a striking rise. Both supplementation of culture media with LIF and transfection of the ADSCs with miR-302 family upregulated the expression levels of OCT4 , Nanog , and Sox2 mRNAs. These findings showed that mouse adipose tissue contains a population of cells with molecular resemblance to embryonic stem cells, and LIF and miR-302 family positively affect the expression of pluripotency markers.

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