Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity | Zendy

Sonal Gupta | Zendy; Rakhi Bansal | Zendy; Javed Ali | Zendy; Reema Gabrani | Zendy; Shweta Dang | Zendy

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Development and Characterization of Polyphenon 60 and Caffeine Microemulsion for Enhanced Antibacterial Activity

Author(s) -

Sonal Gupta,

Rakhi Bansal,

Javed Ali,

Reema Gabrani,

Shweta Dang

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/932017

Subject(s) - caffeine , antibacterial activity , minimum inhibitory concentration , broth microdilution , dpph , mtt assay , cytotoxicity , chemistry , microemulsion , food science , antimicrobial , traditional medicine , biology , antioxidant , bacteria , in vitro , biochemistry , medicine , organic chemistry , pulmonary surfactant , genetics , endocrinology

Green tea catechins and caffeine have exhibited antibacterial activity; however, their use is limited by lack of stability and effective delivery systems. Polyphenon 60 (P60) and caffeine were encapsulated in a single microemulsion (ME) formulation with an objective to lower the minimum inhibitory concentrations (MICs) of the individual agents against selected pathogens ( S. epidermidis and E. coli ). Combination of two natural compounds would advocate two different mechanisms on the bacterial growth thereby providing for better antibacterial activity. Thermodynamically stable ME was developed and characterized with an average particle size of 17.58 nm, further confirmed by TEM analysis. Antibacterial studies included chequerboard microdilution assay to determine the MIC and fractional inhibitory concentration (FIC) of both the natural compounds individually and in combination. MIC and FIC results indicated that the combination of the above two natural compounds was proficient in lowering the MICs of individual agents. Results of DPPH assay indicated that ME system preserved the long term antioxidative potential of P60 and caffeine. The cytotoxicity of the optimized formulation on Vero cell line by MTT assay was found to be nontoxic to mammalian cells.

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