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Glioblastoma multiforme (GBM) is the most malignant and invasive human brain tumor that is difficult to treat and has a very poor prognosis. Thus, new therapeutic strategies that target GBM are urgently needed. The PI3K/AKT/PTEN signaling pathway is frequently deregulated in a wide range of cancers. The present study was designed to examine the inhibitory effect of  AKT3  or  PI3KCA  siRNAs on GBM cell growth, viability, and proliferation.T98G cells were transfected with  AKT3  and/or  PI3KCA  siRNAs. AKT3 and PI3KCA protein-positive cells were identified using FC and Western blotting. The influence of specific siRNAs on T98G cell viability, proliferation, cell cycle, and apoptosis was evaluated as well using FC. Alterations in the mRNA expression of  AKT3 ,  PI3KCA , and apoptosis-related genes were analyzed using QRT-PCR. Knockdown of  AKT3  and/or  PI3KCA  genes in T98G cells led to a significant reduction in cell viability, the accumulation of subG1-phase cells and, a reduced fraction of cells in the S and G2/M phases. Additionally, statistically significant differences in the BAX/BCL-2 ratio and an increased percentage of apoptotic cells were found. The siRNA-induced  AKT3  and  PI3KCA  mRNA knockdown may offer a novel therapeutic strategy to control the growth of human GBM cells.

biomed research international

Knockdown ofAKT3(PKBγ) andPI3KCASuppresses Cell Viability and Proliferation and Induces the Apoptosis of Glioblastoma Multiforme T98G Cells