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Genotypes of  Mycobacterium tuberculosis  complex (MTBC) vary with the geographic origin of the patients and can affect tuberculosis (TB) transmission. This study was aimed to further differentiate spoligotype-defined clusters of drug-resistant MTBC clinical isolates split in Beijing ( n  = 190) versus non-Beijing isolates ( n  = 84) from Sichuan region, the second high-burden province in China, by IS 6110 -restriction fragment length polymorphism (RFLP) and 24-locus MIRU-VNTRs. Among 274 spoligotyped isolates, the clustering ratio of Beijing family was 5.3% by 24-locus MIRU-VNTRs versus 2.1% by IS 6110 -RFLP, while none of the non-Beijing isolates were clustered by 24-locus MIRU-VNTRs versus 9.5% by IS 6110 -RFLP. Hence, neither the 24-locus MIRU-VNTR was sufficient enough to fully discriminate the Beijing family, nor the IS 6110 -RFLP for the non-Beijing isolates. A region adjusted scheme combining 12 highly discriminatory VNTR loci with IS 6110 -RFLP was a better alternative for typing Beijing strains in Sichuan than 24-locus MIRU-VNTRs alone. IS 6110 -RFLP was for the first time introduced to systematically genotype MTBC in Sichuan and we conclude that the region-adjusted scheme of 12 highly discriminative VNTRs might be a suitable alternative to 24-locus MIRU-VNTR scheme for non-Beijing strains, while the clusters of the Beijing isolates should be further subtyped using IS 6110 -RFLP for optimal discrimination.

biomed research international

Suitability of IS<i>6110</i>-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-Resistant<i>Mycobacterium tuberculosis</i>Clinical Isolates from Sichuan in China

Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-ResistantMycobacterium tuberculosisClinical Isolates from Sichuan in China