SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool
Author(s) -
Santosh Kumar Upadhyay,
Shailesh Sharma
Publication year - 2014
Publication title -
biomed research international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 126
eISSN - 2314-6141
pISSN - 2314-6133
DOI - 10.1155/2014/742482
Subject(s) - crispr , python (programming language) , computer science , palindrome , computational biology , genome editing , cas9 , throughput , genome , biology , genetics , operating system , gene , wireless
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online.
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