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The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of  Manihot esculenta ), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to  Ascomycota  phylum. The strain  Aspergillus  6V4 was the best amylase producer in the screening  assay of starch hydrolysis in petri dishes (ASHPD)  and in the  assay in submerged fermentation (ASbF) . The strain  Aspergillus  6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of  Aspergillus  6V4 amylases (385 IU/g) in solid state fermentation (SSF).

Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate