Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate | Zendy

Jessyca dos Reis Celestino | Zendy; Ana Caroline Duarte | Zendy; Cláudia Maria de Melo Silva | Zendy; Hellen Holanda Sena | Zendy; Maria do Perpétuo Socorro Borges Carriço Ferreira | Zendy; Neila Hiraishi Mallmann | Zendy; Natacha Pinheiro Costa Lima | Zendy; Chanderlei de Castro Tavares | Zendy; Rodrigo Otávio Silva de Souza | Zendy; Érica Simplício de Souza | Zendy; João Vicente Braga de Souza | Zendy

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Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate

Author(s) -

Jessyca dos Reis Celestino,

Ana Caroline Duarte,

Cláudia Maria de Melo Silva,

Hellen Holanda Sena,

Maria do Perpétuo Socorro Borges Carriço Ferreira,

Neila Hiraishi Mallmann,

Natacha Pinheiro Costa Lima,

Chanderlei de Castro Tavares,

Rodrigo Otávio Silva de Souza,

Érica Simplício de Souza,

João Vicente Braga de Souza

Publication year - 2014

Publication title -

enzyme research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.439

H-Index - 39

eISSN - 2090-0406

pISSN - 2090-0414

DOI - 10.1155/2014/725651

Subject(s) - amylase , bran , solid state fermentation , food science , fermentation , aspergillus , aspergillus oryzae , starch , maltose , strain (injury) , substrate (aquarium) , hydrolysis , penicillium , biology , enzyme , chemistry , microbiology and biotechnology , biochemistry , sucrose , raw material , ecology , anatomy

The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta ), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF) . The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF).

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