Radiosensitization Effect of Nedaplatin on Nasopharyngeal Carcinoma Cells in Different Status of Epstein-Barr Virus Infection | Zendy

Li Yin | Zendy; Jing Wu | Zendy; Jianfeng Wu | Zendy; Jinjun Ye | Zendy; Xuesong Jiang | Zendy; Meng Chen | Zendy; Dejun Wang | Zendy; Xue Wang | Zendy; Dan Zong | Zendy; Jiajia Gu | Zendy; Junying Zhang | Zendy; Jian Wu | Zendy; Lin Xu | Zendy; Xia He | Zendy; Wenjie Guo | Zendy

Open Access

Radiosensitization Effect of Nedaplatin on Nasopharyngeal Carcinoma Cells in Different Status of Epstein-Barr Virus Infection

Author(s) -

Li Yin,

Jing Wu,

Jianfeng Wu,

Jinjun Ye,

Xuesong Jiang,

Meng Chen,

Dejun Wang,

Xue Wang,

Dan Zong,

Jiajia Gu,

Junying Zhang,

Jian Wu,

Lin Xu,

Xia He,

Wenjie Guo

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/713674

Subject(s) - nedaplatin , nasopharyngeal carcinoma , cell cycle , radiosensitivity , apoptosis , cell culture , cytotoxicity , chemistry , flow cytometry , cell cycle checkpoint , cancer research , mtt assay , cell , biology , immunology , in vitro , medicine , chemotherapy , cisplatin , biochemistry , radiation therapy , genetics

This study aims to evaluate the radiosensitization effect of nedaplatin on nasopharyngeal carcinoma (NPC) cell lines with different Epstein-Barr virus (EBV) status. Human NPC cell lines CNE-2 (EBV-negative) and C666 (EBV-positive) were treated with 0–100 μ g/mL nedaplatin, and inhibitory effects on cell viability and IC 50 were calculated by MTS assay. We assessed changes in radiosensitivity of cells by MTS and colony formation assays, and detected the apoptosis index and changes in cell cycle by flow cytometry. MTS assay showed that nedaplatin caused significant cytotoxicity in CNE-2 and C666 cells in a time- and dose-dependent manner. After 24 h, nedaplatin inhibited growth of CNE-2 and C666 cells with IC 50 values of 34.32 and 63.69 μ g/mL, respectively. Compared with radiation alone, nedaplatin enhanced the radiation effect on both cell lines. Nedaplatin markedly increased apoptosis and cell cycle arrest in G2/M phase. Nedaplatin radiosensitized human NPC cells CNE-2 and C666, with a significantly greater effect on the former. The mechanisms of radiosensitization include induction of apoptosis and enhancement of cell cycle arrest in G2/M phase.

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