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Three culture media ( Brucella  agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating  Brucella  spp. One hundred lymph nodes from pigs ( n  = 50) and wild boars ( n  = 50) with lymphadenitis were collected in slaughterhouses in the State of São Paulo and were assessed on these three selective media for  Brucella  spp. All of the samples were negative for  Brucella  spp. on the three culture media. On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media. The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than the  Brucella  agar.

biomed research international

Evaluation of Three Formulations of Culture Media for Isolation of<i>Brucella</i>spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms

Evaluation of Three Formulations of Culture Media for Isolation ofBrucellaspp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms