Evaluation of Three Formulations of Culture Media for Isolation ofBrucellaspp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms | Zendy

Acácia Ferreira Vicente | Zendy; J. M. A. P. Antunes | Zendy; Gustavo Henrique Batista Lara | Zendy; Mateus de Souza Ribeiro Mioni | Zendy; Susan Dora Allendorf | Zendy; Marina Gea Peres | Zendy; Camila Michele Appolinário | Zendy; Fernando José Paganini Listoni | Zendy; Márcio Garcia Ribeiro | Zendy; Jane Megid | Zendy

Open Access

Evaluation of Three Formulations of Culture Media for Isolation ofBrucellaspp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms

Author(s) -

Acácia Ferreira Vicente,

J. M. A. P. Antunes,

Gustavo Henrique Batista Lara,

Mateus de Souza Ribeiro Mioni,

Susan Dora Allendorf,

Marina Gea Peres,

Camila Michele Appolinário,

Fernando José Paganini Listoni,

Márcio Garcia Ribeiro,

Jane Megid

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/702072

Subject(s) - brucella , agar , microbiology and biotechnology , brucella abortus , agar plate , isolation (microbiology) , biology , bacteria , brucellosis , microbiological culture , veterinary medicine , food science , medicine , immunology , genetics

Three culture media ( Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating Brucella spp. One hundred lymph nodes from pigs ( n = 50) and wild boars ( n = 50) with lymphadenitis were collected in slaughterhouses in the State of São Paulo and were assessed on these three selective media for Brucella spp. All of the samples were negative for Brucella spp. on the three culture media. On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media. The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than the Brucella agar.

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