Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation | Zendy

Chin-Chen Chen | Zendy; Jiun-Tsai Lin | Zendy; Yi-Fang Cheng | Zendy; Cheng-Yi Kuo | Zendy; Chun-Fang Huang | Zendy; ShaoHsuan Kao | Zendy; Yao-Jen Liang | Zendy; ChingYi Cheng | Zendy; Han-Min Chen | Zendy

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Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation

Author(s) -

Chin-Chen Chen,

Jiun-Tsai Lin,

Yi-Fang Cheng,

Cheng-Yi Kuo,

Chun-Fang Huang,

ShaoHsuan Kao,

Yao-Jen Liang,

ChingYi Cheng,

Han-Min Chen

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/692061

Subject(s) - ampk , inflammation , microglia , nitric oxide synthase , nitric oxide , chemistry , protein kinase a , amp activated protein kinase , iκbα , proinflammatory cytokine , nf κb , microbiology and biotechnology , phosphorylation , signal transduction , medicine , biochemistry , biology , organic chemistry

Adenosine 5′-monophosphate-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis via modulating metabolism of glucose, lipid, and protein. In addition to energy modulation, AMPK has been demonstrated to associate with several important cellular events including inflammation. The results showed that ENERGI-F704 identified from bamboo shoot extract was nontoxic in concentrations up to 80 μ M and dose-dependently induced phosphorylation of AMPK (Thr-172) in microglia BV2 cells. Our findings also showed that the treatment of BV2 with ENERGI-F704 ameliorated the LPS-induced elevation of IL-6 and TNF- α production. In addition, ENERGI-F704 reduced increased production of nitric oxide (NO) and prostaglandin E2 (PGE2) via downregulating the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2), respectively. Moreover, ENERGI-F704 decreased activated nuclear translocation and protein level of NF- κ B. Inhibition of AMPK with compound C restored decreased NF- κ B translocation by ENERGI-F704. In conclusion, ENERGI-F704 exerts inhibitory activity on LPS-induced inflammation through manipulating AMPK signaling and exhibits a potential therapeutic agent for neuroinflammatory disease.

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