A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus | Zendy

Chun-Hsien Tseng | Zendy; HsiangJung Tsai | Zendy; Chung-Ming Chang | Zendy

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A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

Author(s) -

Chun-Hsien Tseng,

HsiangJung Tsai,

Chung-Ming Chang

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/653056

Subject(s) - subtyping , neuraminidase , influenza a virus subtype h5n1 , virology , hemagglutinin (influenza) , biology , influenza a virus , virus , genome , avian influenza virus , h5n1 genetic structure , identification (biology) , computational biology , medicine , genetics , gene , covid-19 , disease , computer science , pathology , infectious disease (medical specialty) , botany , programming language

. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method . This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. Result and Conclusion . This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

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