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Increased frequency of methicillin-resistant  Staphylococcus aureus  (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A ( spa ) and coagulase ( coa ) gene polymorphisms to verify their ability in assessing the relatedness of isolates. Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibiotyping and PCR-RFLP analysis of  coa  and  spa  genes. Thirty-two antibiotypes were identified.  coa  gene PCR generated 3 types and 10 subtypes of band patterns.  HaeIII  restriction digestion of amplified  coa  gene products produced 5 major banding patterns and 12 subtypes.  spa  gene PCR products generated 4 major and 11 minor types, and their  HaeII  restriction digestion showed 5 major and 12 minor banding patterns. The combined  coa  and  spa  RFLP patterns generated 22 combined R types. Typing using  coa  PCR and PCR-RFLP had the same discriminatory index (DI) value (0.64), which was comparable to that of both  spa  PCR and PCR-RFLP techniques (0.68). The combined grouping increased the DI value to 0.836. The current study revealed that testing for multiple gene polymorphisms is more useful for local epidemiologic purposes.

Molecular Typing of Methicillin ResistantStaphylococcus aureusClinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms