English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This paper describes a comparative study of the decomposition of two nitrosyl iron complexes (NICs) with penicillamine thiolic ligands [Fe 2 (SC 5 H 11 NO 2 ) 2 (NO) 4 ]SO 4   · 5H 2 O ( I ) and glutathione- (GSH-) ligands [Fe 2 (SC 10 H 17 N 3 O 6 ) 2 (NO) 4 ]SO 4   · 2H 2 O ( II ), which spontaneously evolve to NO in aqueous medium. NO formation was measured by a sensor electrode and by spectrophotometric methods by measuring the formation of a hemoglobin- (Hb-) NO complex. The NO evolution reaction rate from ( I )   k   1  = (4.6 ± 0.1) · 10 −3  s −1  and the elimination rate constant of the penicillamine ligand  k   2  = (1.8 ± 0.2) · 10 −3  s −1  at 25°C in 0.05 M phosphate buffer,  pH 7.0, was calculated using kinetic modeling based on the experimental data. Both reactions are reversible. Spectrophotometry and mass-spectrometry methods have firmly shown that the penicillamine ligand is exchanged for GS −  during decomposition of 1.5 · 10 −4  M ( I ) in the presence of 10 −3  M GSH, with 76% yield in 24 h. As has been established, such behaviour is caused by the resistance of ( II ) to decomposition due to the higher affinity of iron to GSH in the complex. The discovered reaction may impede S-glutathionylation of the essential enzyme systems in the presence of ( I ) and is important for metabolism of NIC, connected with its antitumor activity.

Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine