Differential Expression of Cholecystokinin A Receptor in Gallbladder Cancer in the Young and Elderly Suggests Two Subsets of the Same Disease? | Zendy

Hasan Raza Kazmi | Zendy; Abhijit Chandra | Zendy; Kavita Baghel | Zendy; Anshuman Singh | Zendy; Jaya Nigam | Zendy; Devendra Parmar | Zendy; Abbas Ali Mahdi | Zendy; Sudhir K. Goel | Zendy; Sandeep Kumar | Zendy

Open Access

Differential Expression of Cholecystokinin A Receptor in Gallbladder Cancer in the Young and Elderly Suggests Two Subsets of the Same Disease?

Author(s) -

Hasan Raza Kazmi,

Abhijit Chandra,

Kavita Baghel,

Anshuman Singh,

Jaya Nigam,

Devendra Parmar,

Abbas Ali Mahdi,

Sudhir K. Goel,

Sandeep Kumar

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/625695

Subject(s) - cholecystokinin , disease , gallbladder , gallbladder cancer , cancer , medicine , receptor , differential diagnosis , differential (mechanical device) , gallbladder disease , biology , cancer research , bioinformatics , oncology , pathology , engineering , aerospace engineering

Background . Cholecystokinin type A receptor (CCKAR) is known to be overexpressed in variety of human malignancies but information regarding its expression in gallbladder cancer (GBC) is limited. Attempts were now made to investigate expression pattern of CCKAR mRNA and protein in controls and GBC patients and correlate it with various clinicopathological parameters following surgical resection. Materials and Methods . Gallbladder tissue samples from 64 subjects (GBC: 39; control: 25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-polymerase chain reaction and confirmed using real-time polymerase chain reaction. Protein expression was studied by enzyme-linked immunosorbent assay. Results . Significantly higher expression of CCKAR mRNA ( P < 0.0001) and protein ( P < 0.0001) was observed in GBC tissues. Overexpression was also observed for stage III and in moderately and poorly differentiated tumors. When the clinicopathological parameters were compared, we found age dependent decrease in CCKAR expression. Relatively higher expression of CCKAR was observed in younger patients (age < 45 years) having more aggressive disease when compared with elderly ones (age ≥ 45 years). Conclusions . Age related differential expression of CCKAR in GBC may suggest two possible variants of the disease in this endemic belt.

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