Production and Characterization of a Polyclonal Antibody of Anti-rLipL21-IgG againstLeptospirafor Early Detection of Acute Leptospirosis | Zendy

Arivudainambi Seenichamy | Zendy; Abdul Rani Bahaman | Zendy; Abdul Rahim Mutalib | Zendy; S. KhairaniBejo | Zendy

Open Access

Production and Characterization of a Polyclonal Antibody of Anti-rLipL21-IgG againstLeptospirafor Early Detection of Acute Leptospirosis

Author(s) -

Arivudainambi Seenichamy,

Abdul Rani Bahaman,

Abdul Rahim Mutalib,

S. KhairaniBejo

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/592858

Subject(s) - leptospirosis , polyclonal antibodies , leptospira , antibody , leptospira interrogans , serotype , microbiology and biotechnology , biology , virology , antigen , recombinant dna , immunoglobulin g , immunology , gene , biochemistry

Leptospirosis is one of the zoonotic diseases in animals and humans throughout the world. LipL21 is one of the important surface-exposed lipoproteins in leptospires and the most effective cross protective immunogenic antigen. It is widely considered as a diagnostic marker for leptospirosis. In this study, we evaluated the serodiagnostic potential of LipL21 protein of Leptospira interrogans serovar Pomona. We have successfully amplified, cloned, and expressed LipL21 in E. coli and evaluated its specificity by immunoblotting. Purified recombinant LipL21 (rLipL21) was inoculated into rabbits for the production of polyclonal antibody. Characterization of the purified IgG antibody against rLipL21 was performed by cross reactivity assay. Only sera from leptospirosis patients and rabbit hyperimmune sera recognized rLipL21 while the nonleptospirosis control sera showed no reaction in immunoblotting. We confirmed that anti-rLipL21-IgG antibody cross reacted with and detected only pathogenic leptospiral species and it did not react with nonpathogenic leptospires and other bacterial species. Results observed showed that anti-rLipL21-IgG antibody has high specificity and sensitivity to leptospires. The findings indicated that the antibody could be used in a diagnostic assay for detection of leptospires or their proteins in the early phase of infection.

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