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The literature has identified complex aspects of intracellular host-parasite relationships, which require systematic, nonreductionist approaches and spatial/temporal information. Increasing and integrating temporal and spatial dimensions in host cell imaging have contributed to elucidating several conceptual gaps in the biology of intracellular parasites. To access and investigate complex and emergent dynamic events, it is mandatory to follow them in the context of living cells and organs, constructing scientific images with integrated high quality spatiotemporal data. This review discusses examples of how advances in microscopy have challenged established conceptual models of the intracellular life cycles of  Leishmania  spp. and  Trypanosoma cruzi  protozoan parasites.

biomed research international

An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the<i>Leishmania</i>Spp. and<i>Trypanosoma cruzi</i>Host-Parasite Relationship

An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding theLeishmaniaSpp. andTrypanosoma cruziHost-Parasite Relationship